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. 2010 Aug 18;30(33):10977–10984. doi: 10.1523/JNEUROSCI.1077-10.2010

Figure 3.

Figure 3.

TBS-induced increases in spine pPAK are absent in Fmr1-KOs. A, Photomicrographs show similar punctate immunoreactivity for pPAK and PSD95 (and merged) in CA1 str. radiatum of control WT and Fmr1-KO hippocampal slices. Scale bar, 10 μm. B, Photomicrographs of a single synapse containing pPAK (red) and PSD95 (green) immunoreactivities displayed (top to bottom) in 90° clockwise turns. Scale bar, 1 μm. C, Photomicrographs show pPAK immunolabeling in WT slices harvested 7 min after receiving control stimulation (Con) or TBS. D, Bar graph shows quantification of pPAK-immunopositive (+) spines (double-labeled for PSD95) in str. radiatum of WT and Fmr1-KO slices that received control stimulation or TBS and collected at the indicated postinduction time points; only the WT slices had elevated numbers of pPAK+ spines at 7 min post-TBS (*p = 0.014 vs WT control; +p = 0.035 vs Fmr1-KO, 7 min post-TBS group; n > 8 slices/group).