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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Osteoarthritis Cartilage. 2010 Jul 13;18(9):1203–1210. doi: 10.1016/j.joca.2010.05.016

Fig. 2. p38α is localized to the nucleus and p38γ to the cytosol.

Fig. 2

Chondrocytes were stimulated with IL-1β or Fibronectin fragment (FN-f) for 5 and 30 minutes. Cytosolic and nuclear fractions were immunoblotted with antibodies specific for total p38α or p38γ isoforms. Blots were stripped and immunoblotted with Lamin B as a nuclear marker protein to confirm separation of nuclear from cytosolic proteins. Results are representative of three independent experiments.