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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Osteoarthritis Cartilage. 2010 Jul 13;18(9):1203–1210. doi: 10.1016/j.joca.2010.05.016

Fig. 3. Effects of the p38 inhibitors SB203580 and BIRB796 on p38α and p38γ activity.

Fig. 3

(A) Chondrocytes were pretreated with the indicated doses of SB203580 or BIRB796 prior to stimulation with IL-1β. Lysates were then immunoblotted with phosphospecific HSP27 antibody. (B) Immunoblots from two independent experiments were scanned and densitometric analysis was performed to determine intensity of the phosphorylated HSP27 band. Results are means and 95% confidence intervals. (C) Chondrocytes were pretreated with 10µM SB203580 or 0.5µM BIRB796 prior to IL-1β stimulation. Lysates were measured with an ELISA for phosphorylated p38γ. Results are mean and 95% confidence intervals of three independent experiments. (D) Chondrocytes were pretreated with 10µM SB203580 or 0.5µM BIRB796 prior to IL-1β stimulation. Lysates were immunopreciptated with p38γ specific antibody. Immunoprecipitates were then used in an in vitro kinase assay using recombinant ATF-2 as a substrate.