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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Osteoarthritis Cartilage. 2010 Jul 13;18(9):1203–1210. doi: 10.1016/j.joca.2010.05.016

Fig. 4. Effects of the p38 inhibitors SB203580 and BIRB796 on IL-1 and FN-f stimulation of MMP-13.

Fig. 4

Chondrocytes were pretreated with either 10µM SB203580 or 500nM BIRB796 for 30 minutes prior to overnight stimulation with either 10ng/mL IL-1β or 500nM Fibronectin fragment. (A) Media was collected and an ELISA was run for pro-MMP-13 (n=5, mean±95% confidence intervals) or media was immunblotted with MMP-13 antibody (B). (C) RNA was harvested in parallel to media collection and real time PCR was run with MMP-13 primers in duplicate samples. Results were normalized to GAPDH as a housekeeping gene (n=2, mean±95% confidence intervals).