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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Osteoarthritis Cartilage. 2010 Jul 13;18(9):1203–1210. doi: 10.1016/j.joca.2010.05.016

Fig. 5. Effects on chondrocyte MMP-13 production after overexpression of constitutively active and dominant negative p38γ.

Fig. 5

(A) Osteoarthritic chondrocytes were transfected with 3µg constitutively active (CA) HA-tagged p38γ plasmid or empty plasmid vector as a control. 48 hours after transfection, media was collected and pro-MMP-13 was measured by an ELISA. Results are the mean±95% confidence intervals of 3 independent experiments. (B) Normal human chondrocytes were transfected with 3µg constitutively active (CA) p38γ plasmid or empty plasmid vector as a control. 48 hours after transfection cells were stimulated with either 10ng/mL IL-1β or 500nM fibronectin fragment overnight. Media was collected and MMP-13 was measured by immunoblotting. Bands from the IL-1β experiment were from the same gel run at the same time and were spliced together (indicated by a line) due to being in noncontiguous lanes. (C) Chondrocytes were infected with adenovirus expressing dominant negative p38γ or non-expressing adenovirus as a control. 48 hours later cells were stimulated with either 10ng/mL IL-1β or 500nM Fibronectin fragment overnight. Media was collected and MMP-13 was measured by ELISA. Results are the mean and 95% confidence intervals of 3 independent experiments for IL-1β and 2 experiments for Fn-f.