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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Breast Cancer Res Treat. 2009 Dec 25;124(1):27–38. doi: 10.1007/s10549-009-0699-0

Fig. 7.

Fig. 7

Effects of PRSS3 knockdown and CD109 knockdown on the growth morphology and cell polarization of T4-2 cells in 3D culture. Cells transduced with control lentiviral shRNA vector (NT) or with PRSS3-targeting vector C24 (PRSS3 kd) or CD109-targeting vector (CD109 kd) were cultured in Matrigel for 7 days. Knockdown of PRSS3 or CD109 suppressed disorganized growth and led to formation of acini with basal polarity, as demonstrated by immunofluorescence staining for E-cadherin (a–c) and α6-integrin (d–f). Scale bar, 25 µm. g Quantitative analysis of polarity by percentage of colonies with polarized distribution of basal α6-integrin confirmed that a significantly greater proportion of colonies in cultures with PRSS3 and CD109 knockdown showed basal polarity. Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01 (unpaired t test)