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. 2001 Feb 6;98(4):1543–1548. doi: 10.1073/pnas.041588198

Figure 5.

Figure 5

Me180 cells expressing Stat3-C contain elevated expression of Bcl-xL and survivin, and decreased expression of Bax. (A) Northern blot analysis of Bcl-xL on total RNA prepared from Me180 cells (Me) or Me180 cells stably expressing Stat3C (Me 3-C) treated with IFN-γ for 4 h (IFN-γ) or untreated (−). Blot was reprobed with GAPDH to control equal loading. (B) Western blot analysis of Bcl-2, Bax, and survivin. Fifty micrograms of whole cell extract from Me180 cells stably transfected with the control plasmid [Me(Rc)] or Me180 cells expressing Stat3C (Me 3-C) treated with IFN-γ (18 ng/ml) for 24 h (IFN-γ) or untreated (−) were blotted with the indicated antibody.