Fig. 2.

Effects of scFv173 on adhesion of cells to extracellular matrix proteins. Adhesion assay of cells (HTB-182, EKVX, OVCAR-3 or SKOV-3) preincubated with antibodies and seeded in 96-well tissue culture plates coated with laminin. The cells were allowed to adhere for 1 h before half of the wells were washed twice with PBS and the number of cells in each well quantified using MTS. Inhibition of cell adhesion to laminin is represented as the ratio of MTS measurement in wells washed with PBS and wells that were not washed. Black bars shows adhesion at 4°C and white bars adhesion at 37°C. For comparison the ratio of control wells without antibodies (scFv173 or anti-ALCAM from RnD systems) added is 100%. Five independent experiments, each with triplicate wells