Fig. 3.

Effects of scFv173 on MDA-MB-231 cell viability, adhesion and Matrigel invasion assay. a Breast cancer MDA-MB-231 cells were added antibody, scFv173 or scFv141, to a final concentration of 1 μM (black bar) or 1 nM (white bar) and the cell viability measured after 48 h in a MTS assay. Control cells were grown with ordinary medium or added gambogic acid (GA) known to induce apoptosis in tumour cell lines. b Cell adhesion to laminin at 4°C as previously described using MDA-MB-231 cells. c ³H-thymidine labelled MDA-MB-231 cells were mixed with antibodies prior to seeding and harvested after 48 h. The ratio of invading cells over non-invading cells in treated wells are normalised to the ratio in untreated wells. The bars represents the average with standard deviation of 2 (a, b) or 3 (c) independent experiments with triplicate wells for each treatment. p values were determined using a two tailed t test comparing controls and scFv173-treated cells or controls and anti-ALCAM treated cells