Figure 3. DEET-induced action potentials required Gr66a, Gr33a and Gr32a.

(A) Tip recordings showing the mean responses in s6 sensilla to 0.2% DEET. The deficits in the Gr66a^ex83, Gr33a¹ and ΔGr32a mutants were rescued significantly by the wild-type transgenes. The rescue control for Gr66a was 7-Gr66a⁻ and the rescue transgene was 8-Gr66a⁺. Gr66a^ex stands for Gr66a^ex83. The Gr33a rescue construct was UAS-Gr33a⁺ and the Gr32a rescue was performed using the genomic transgene, gGr32a⁺. The error bars indicate S.E.M.s. (B) Representative traces of DEET-induced action potentials in the wild-type control, Gr33a¹ and Gr33a expressing the wild-type transgene: Gr33a¹/Gr33a^GAL4;UAS-Gr33a/+. The times when the recording pipets were applied to the s6 sensilla are indicated by the asterisks. Arrowheads indicate DEET-induced action potentials.