Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1967 Dec;46(12):1883–1893. doi: 10.1172/JCI105678

The Mechanism of Appearance of Immunoglobulin A in Nasal Secretions in Man*

William T Butler 1,2,‡, Roger D Rossen 1,2, Thomas A Waldmann 1,2
PMCID: PMC292941  PMID: 4169362

Abstract

Experiments were done to investigate mechanisms of appearance of both 7 S and 11 S IgA in nasal secretions. Three IgA preparations, (a) 11 S IgA from nasal secretions, (b) 7 S IgA from homologous serum, and (c) 7 S IgA from autologous serum, were isolated, labeled, and injected intravenously into volunteers. The rate of disappearance from plasma and the extent and nature of their appearance in nasal secretions were examined in detail. After intravenous injection, 11 S IgA from nasal secretions disappeared rapidly from plasma. However, only negligible amounts of 11 S IgA were detected in nasal secretion, which suggested that rapid selective secretion of circulating 11 S IgA does not occur as a mechanism of IgA accumulation in nasal secretions. Both the homologous and autologous 7 S IgA preparations disappeared from plasma at a normal rate, and both appeared in nasal secretions unchanged in sedimentation behavior. The specific activity of IgA in nasal secretions, when related to the total IgA concentration, was about 30-fold less than that in serum. When related to only the 7 S IgA concentration of nasal secretions, the specific activity was about one-half that of serum. These studies are consistent with the following hypotheses: (a) circulating 7 S IgA is a source of part of the 7 S IgA found in nasal secretions. The remainder of the nasal secretion 7 S IgA may be synthesized locally in the tissues of the upper respiratory tract; (b) 11 S IgA in nasal secretions is not assembled from serum 7 S IgA components; and (c) 11 S IgA in nasal secretions is synthesized de novo in the tissues of the upper respiratory tract.

Full text

PDF
1887

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARTENSTEIN M. S., BELLANTI J. A., BUESCHER E. L. IDENTIFICATION OF THE ANTIVIRAL SUBSTANCES IN NASAL SECRETIONS. Proc Soc Exp Biol Med. 1964 Nov;117:558–564. doi: 10.3181/00379727-117-29637. [DOI] [PubMed] [Google Scholar]
  2. Alford R. H., Rossen R. D., Butler W. T., Kasel J. A. Neutralizing and hemagglutination-inhibiting activity of nasal secretions following experimental human infection with A2 influenza virus. J Immunol. 1967 Apr;98(4):724–731. [PubMed] [Google Scholar]
  3. BELLANTI J. A., ARTENSTEIN M. S., BUESCHER E. L. CHARACTERIZATION OF VIRUS NEUTRALIZING ANTIBODIES IN HUMAN SERUM AND NASAL SECRETIONS. J Immunol. 1965 Mar;94:344–351. [PubMed] [Google Scholar]
  4. CHODIRKER W. B., TOMASI T. B., Jr GAMMA-GLOBULINS: QUANTITATIVE RELATIONSHIPS IN HUMAN SERUM AND NONVASCULAR FLUIDS. Science. 1963 Nov 22;142(3595):1080–1081. doi: 10.1126/science.142.3595.1080. [DOI] [PubMed] [Google Scholar]
  5. Cate T. R., Rossen R. D., Douglas R. G., Jr, Butler W. T., Couch R. B. The role of nasal secretion and serum antibody in the rhinovirus common cold. Am J Epidemiol. 1966 Sep;84(2):352–363. doi: 10.1093/oxfordjournals.aje.a120648. [DOI] [PubMed] [Google Scholar]
  6. Douglas R. G., Jr, Rossen R. D., Butler W. T., Couch R. B. Rhinovirus neutralizing antibody in tears, parotid saliva, nasal secretions and serum. J Immunol. 1967 Aug;99(2):297–303. [PubMed] [Google Scholar]
  7. Hong R., Pollara B., Good R. A. A model for colostral IgA. Proc Natl Acad Sci U S A. 1966 Aug;56(2):602–607. doi: 10.1073/pnas.56.2.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Masson P. L., Heremans J. F., Prignot J. Studies on the proteins of human bronchial secretions. Biochim Biophys Acta. 1965 Dec 16;111(2):466–478. doi: 10.1016/0304-4165(65)90056-5. [DOI] [PubMed] [Google Scholar]
  9. McFARLANE A. S. Efficient trace-labelling of proteins with iodine. Nature. 1958 Jul 5;182(4627):53–53. doi: 10.1038/182053a0. [DOI] [PubMed] [Google Scholar]
  10. McFarlin D. E., Strober W., Wochner R. D., Waldmann T. A. Immunoglobulin A production in ataxia telangiectasia. Science. 1965 Nov 26;150(3700):1175–1177. doi: 10.1126/science.150.3700.1175. [DOI] [PubMed] [Google Scholar]
  11. REMINGTON J. S., VOSTI K. L., LIETZE A., ZIMMERMAN A. L. SERUM PROTEINS AND ANTIBODY ACTIVITY IN HUMAN NASAL SECRETIONS. J Clin Invest. 1964 Aug;43:1613–1624. doi: 10.1172/JCI105037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rossen R. D., Alford R. H., Butler W. T., Vannier W. E. The separation and characterization of proteins intrinsic to nasal secretion. J Immunol. 1966 Sep;97(3):369–378. [PubMed] [Google Scholar]
  13. Rossen R. D., Butler W. T., Cate T. R., Szwed C. F., Couch R. B. Protein composition of nasal secretion during respiratory virus infection. Proc Soc Exp Biol Med. 1965 Aug-Sep;119(4):1169–1176. doi: 10.3181/00379727-119-30406. [DOI] [PubMed] [Google Scholar]
  14. Rossen R. D., Butler W. T., Vannier W. E., Douglas R. G., Jr, Steinberg A. G. The sedimentation and antigenic properties of proteins in nasal and other external secretions. J Immunol. 1966 Dec;97(6):925–938. [PubMed] [Google Scholar]
  15. Rossen R. D., Douglas G., Jr, Cate T. R., Couch R. B., Butler W. T. The sedimentation behavior of rhinovirus neutralizing activity in nasal secretion and serum following the rhinovirus common cold. J Immunol. 1966 Oct;97(4):532–538. [PubMed] [Google Scholar]
  16. Rossen R. D., Schade A. L., Butler W. T., Kasel J. A. The proteins in nasal secretion: a longitudinal study of the gammaA-globulin, gammaG-globulin, albumin, siderophilin, and total protein concentrations in nasal washings from adult male volunteers. J Clin Invest. 1966 May;45(5):768–776. doi: 10.1172/JCI105391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rossen R. D., Wolff S. M., Butler W. T., Vannier W. E. The identification of low molecular weight bentonite flocculating antibodies in the serum of the rabbit, monkey and man. J Immunol. 1967 Apr;98(4):764–777. [PubMed] [Google Scholar]
  18. Smith C. B., Purcell R. H., Bellanti J. A., Chanock R. M. Protective effect of antibody to parainfluenza type 1 virus. N Engl J Med. 1966 Nov 24;275(21):1145–1152. doi: 10.1056/NEJM196611242752101. [DOI] [PubMed] [Google Scholar]
  19. South M. A., Cooper M. D., Wollheim F. A., Hong R., Good R. A. The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. J Exp Med. 1966 Apr 1;123(4):615–627. doi: 10.1084/jem.123.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stiehm E. R., Vaerman J. P., Fudenberg H. H. Plasma infusions in immunologic deficiency states: metabolic and therapeutic studies. Blood. 1966 Dec;28(6):918–937. [PubMed] [Google Scholar]
  21. TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TOMASI T. B., Jr, ZIGELBAUM S. THE SELECTIVE OCCURENCE OF GAMMA-1A GLOBULINS IN CERTAIN BODY FLUIDS. J Clin Invest. 1963 Oct;42:1552–1560. doi: 10.1172/JCI104840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wochner R. D., Drews G., Strober W., Waldmann T. A. Accelerated breakdown of immunoglobulin G (IgG) in myotonic dystrophy: a hereditary error of immunoglobulin catabolism. J Clin Invest. 1966 Mar;45(3):321–329. doi: 10.1172/JCI105346. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES