Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Med. 2010 May 16;16(7):809–813. doi: 10.1038/nm.2154

Figure 3. Treg cells impair effector cell function.

Figure 3

(a) ELISA for human IFN-γ performed on serum samples harvested from mice transplanted with an arterial graft reconstituted with allogeneic PBMC in the presence or absence of ex vivo expanded CD25hi or CD127lo Treg cells. (b) Expression of IFN-γ and FOXP3 mRNA measured by real-time RT-PCR in transplanted grafts from mice inoculated with allogeneic PBMC or allogeneic PBMC and Treg cells (n = 4–6, *P < 0.05 **P < 0.01). (c) Human IFN-γ ELISA on supernatants from cultures of PBMC 5 days after stimulation with irradiated allogeneic PBMC in the presence or absence of Treg cells (* P = 0.0260 ** P = 0.0022). (d) Infiltration of human CD4 and CD8 T cells into the adventitial and neointimal layers of the arterial grafts in mice reconstituted with allogeneic PBMC alone or PBMC and Treg cells. (n = 3 separate experiments; L = lumen). Error bars represent the means ± s.d.