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. Author manuscript; available in PMC: 2011 May 28.
Published in final edited form as: Immunity. 2010 May 6;32(5):654–669. doi: 10.1016/j.immuni.2010.04.011

Figure 3. HIV-1 Traffics via Autophagosomes Prior to Lysosomal Targeting.

Figure 3

(A) Confocal immunofluorescence analysis of HIV-1Gag (green), LC3 (red), and Lamp1 (blue) of DCs pretreated or not with chloroquine (10 μM) and exposed to HIV-1 for indicated times. White arrows indicated colocalization between HIV-1Gag (green) and LC3 (red). Data are representative of three independent experiments.

(B) Magnification of areas of HIV-1Gag colocalizing with LC3+ positive structures indicated by white arrows in (A).

(C) Quantification of autophagosomes (LC3+) colocalizing with HIV-1Gag at each indicated time in presence or absence of chloroquine (Chlq). Data are means of at least 20 cells per condition (±SD) at each time and representative of three independent experiments.

(D) Quantification of lysosomes (Lamp1+) colocalizing with HIV-1Gag at each indicated time in presence or absence of chloroquine (Chlq). Data are means of at least 20 cells (±SD) per condition at each time and representative of three independent experiments.

(E) Quantification of lysosomes (Lamp1+) colocalizing with autophagosomes (LC3+) at each indicated time in presence or absence of chloroquine (Chlq). Data are means of at least 20 cells (±SD) per condition at each time and representative of three independent experiments.

(F–H) The same as in (C)–(E) respectively but during longer time points. Data are means of at least 15 cells and representative of two experiments.

(I) DC exposed to HIV-1 for 120 min and processed as in (A). Z stack confocal analysis was done with slices of 1 μm. The image is from one field out of 20 and representative of three independent experiments.