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. 2001 Jan 30;98(4):1555–1559. doi: 10.1073/pnas.031562298

Figure 1.

Figure 1

Western blot of oocyte plasma membranes. Membranes of 50 oocytes, independently injected with either pSRIImRNA or pHtrIImRNA, have been isolated by subsequent centrifugation over a sucrose and nycodenz density gradient. The blotted membranes have been incubated with an antibody (Qiagen, Hilden, Germany) against the C-terminal 6× histidine-tag of the proteins. The two prominent bands in lanes 1 and 3 at ≈85 kDa and ≈25 kDa can be assigned to pHtrII and pSRII, respectively. Lanes 2 and 4 show control membranes of not injected oocytes.