. Author manuscript; available in PMC: 2011 Jul 28.

Published in final edited form as: J Am Chem Soc. 2010 Jul 28;132(29):10093–10101. doi: 10.1021/ja102632p

Table 1.

Spectroscopic and EPR spin parameters for azurin, its variants, and WT nitrosocyanin

	pH	λ max (nm)	R_L values (^~400nm/_~600nm)	A_z (10⁻⁴ cm⁻¹)	g_z
WT Azurin	5	628	NR	66	2.25
Met121His	7	440, 600	2	102	2.24
Met121Glu	7	450, 615	2.5	83	2.3
Met121Cys	4	451, 627	0.05	54^*	2.26^*
Met121Cys	5	447, 621	0.15	54^*	2.26^*
Met121Cys	6	444, 615	0.23	18^* (65)	2.29^* (2.02)
Met121Cys	7	441, 612	0.29	18^* (65)	2.29^* (2.02)
Met121Cys	8	434, 608	0.55	87^*	2.25^*
Met121Cys	9	430, 591	1.47	87^*	2.25^*
Met121Hcy	7	410, 590	1.5	180	2.25
Nitrosocyanin	7	390, 500	2.5	147	2.24

The parameters are from the main species observed at the pH.

g value and hyperfine splitting in parenthesis are along the x axis.