Skip to main content
. Author manuscript; available in PMC: 2010 Aug 30.
Published in final edited form as: Genes Chromosomes Cancer. 2009 Jan;48(1):83–97. doi: 10.1002/gcc.20620

Figure 4. Analysis of Atp1B2, Dusp2, Smpd3 and FoxJ1 silencing in mouse mammary tumor lines.

Figure 4

A RT-PCR was conducted on cDNA synthesized from indicated mouse mammary tumor lines treated and untreated with 5-azadC/TSA using primers specific for each indicated gene. B. MSP primers specific for each gene were designed and subsequently validated using bisulfite-modified mouse genomic DNA either methylated or not prior to bisulfite conversion. C. MSP analysis of these four genes using DNA harvested from the indicated mouse mammary cell lines was conducted.