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. Author manuscript; available in PMC: 2010 Aug 30.
Published in final edited form as: Genes Chromosomes Cancer. 2009 Jan;48(1):83–97. doi: 10.1002/gcc.20620

Figure 5. Analysis of ATP1B2, DUSP2, SMPD3 and FOXJ1 methylation in human breast tumor cell lines.

Figure 5

A MSP primers were designed to the human ortholog of each indicated gene and validated using human placental DNA that was incubated in the presence or absence of SssI methylase prior to bisulfite conversion. B. MSP analysis of indicated genes was conducted on indicated human breast tumor cell lines. C. Pyrosequencing analysis of the ATP1B2 gene was conducted using genomic DNA harvested from MCF-7, BT-474, and human placenta (control). D. Pyrosequencing analysis of the SMPD3 gene in MDA-MB-231, BT-474 and control DNA. E. Pyrosequencing analysis of the DUSP2 gene in MDA-MB-231, BT-549 and control DNA. F. Pyrosequencing analysis of the FOXJ1 gene in MDA-MB-231, BT-474 and control DNA samples.