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. Author manuscript; available in PMC: 2010 Aug 30.
Published in final edited form as: Genes Chromosomes Cancer. 2009 Jan;48(1):83–97. doi: 10.1002/gcc.20620

Figure 7. Analysis of genes characterized as targets for epigenetic silencing in human breast tumors in a panel of mouse mammary tumor lines.

Figure 7

A MSP primers were designed to the mouse orthologs of the Cdh1, Gstp1, RarB, Wif1, RassF1 and Dapk1 genes. Primer sets were validated using control mouse genomic DNA that was either methylated/unmethylated in vitro prior to bisulfite conversion. B. MSP analysis of indicated genes was conducted on bisulfite-converted DNA isolated from indicated mouse mammary tumor lines. gDNA was harvested from the cell lines and bisulfite-modified. These DNA samples were used in MSP analyses.