Table I.
RT-PCR Primers
| Primer Designation | Human/Mouse | Primer Sequence (5′ ➝ 3′) | Orientation (Forw/Rev) | Annealing Temp (°C) | Thermocycles | Product Size (bp) |
|---|---|---|---|---|---|---|
| Gapdh-RT | M | AACGACCCCTTCATTGAC | F | 57 | 30 | 191 |
| TCCACGACATACTCAGCAC | R | |||||
| Timp3-RT | M | AATTCCTCTGCTCAGGCTCCTGTT | F | 60 | 30 | 429 |
| AGCATGGTCATTTCCTCGGGTTCT | R | |||||
| Rprm-RT | M | TATTCGCCACGCTGCAGACCTAAT | F | 60 | 30 | 359 |
| AAACCGTGCAGACAGGAGCTAAGA | R | |||||
| Smpd3-RT | M | ACATCGATTCTCCCACCAACACCT | F | 60 | 30 | 227 |
| AATTCGCACAATGCAGCTGTCCTC | R | |||||
| Atp1b2-RT | M | TGCTTCTGGAATGTCCTTGACCCT | F | 60 | 30 | 368 |
| TGGGTGGATGAGACCATTTCCTGT | R | |||||
| Dusp2-RT | M | TGGTCCTGTGGAAATCTTGCCCTA | F | 60 | 31 | 307 |
| AATCAGGTATGCCAGGCAGATGGT | R | |||||
| FoxJ1-RT | M | AAGGTAACTTTGACTGGGAGGCCA | F | 60 | 30 | 225 |
| AGGATGTGCCCAAGAAGGTCTCAT | R |