Table III.
Pyrosequencing Primers (Amplification and Sequencing)
| Primer Designation | Human/Mouse | Primer Sequence (5′ ➝ 3′) | Orientation (Forw/Rev) | Annealing Temp (°C) | Thermo- cycles | Product Size (bp) |
|---|---|---|---|---|---|---|
| Rprm-PYRO | M | TTGTAGTTAAGGAAGAAGATGTTAAAAATTAT | F | 50.5 | 50 | 344 |
| AACCTAAAACTTTAAAAAAAAAACTAAAAAAC | R | |||||
| GTTTGGGTGTAGTAG | SEQ* | |||||
| YGTATYGYGYGTTTTAGYGTTTYGTTAT | SEQ to analyze** | |||||
| Timp3-PYRO | M | GATGAATTTTTGTTTAGGTTT | F | 50 | 50 | 195 |
| AAAATCCAAAACTACAAAATAAT | R | |||||
| TTTTTATAAGGATTTGAA | SEQ* | |||||
| YGGTTYGGTYGGTTTYGTTTTGYGTTTYGYGTTTTTYGGTTTYGTT | SEQ to analyze** | |||||
| ATP1B2-PYRO | H | GGTGGGAAGGGGGTGGGTATTT | F | 56 | 50 | 123 |
| CAACCAAAAATAAAAAACCCCCCAAA | R | |||||
| GGGGTGGGTATTTTT | SEQ* | |||||
| YGYGTTYGGYGATYGYGGGTTTTYGTATT | SEQ to analyze** | |||||
| DUSP2-PYRO | H | GAAGTTTTTAGGTTAGTTTAGATTG | F | 53 | 50 | 242 |
| CCTCCAACCCCATAACCACC | R | |||||
| GTTTTTGTATGGTGTTT | SEQ* | |||||
| YGGAGGTYGGTTTAYGGGTATTTAATTYGGGTYGTYGYGGA | SEQ to analyze** | |||||
| FOXJ1-PYRO | H | TTTTAGTTTTAGTTTTTAATTTTAGGTTT | F | 52 | 50 | 290 |
| ACACTTACCACTCTCCCTAAC | R | |||||
| GTTTTAGGAGTAGTTGA | SEQ* | |||||
| YGTTTTAAAGYGTAYGGGATAGGAAGGGGGTGGGGGYGA | SEQ to analyze** | |||||
| SMPD3-PYRO | H | GGATGTGGTTTTGAAGGAGAGATTT | F | 53.5 | 50 | 305 |
| GAATTCAACAACACTAACCTCTA | R | |||||
| GGGTTGTGAGGAG | SEQ* | |||||
| TYGGATTYGAGAGTYGAGAGTYGTYGTYGTTGYGGTYGTYGTT | SEQ to analyze** |
*
SEQ: Sequencing primer
**
SEQ to analyze: Gene-specific sequence analyzed by pyrosequencing (Y: Potential methylation site)