Table V.
Confirmed/Putative TSGs identified in our screen that contain CpG islands in both human and mouse forms of the gene.
| Gene | CpG Island M/H |
RT-PCR* | MSP** M/H |
|---|---|---|---|
| Tspan33 | +/+ | + | −/− |
| Cabc1 | +/+ | + | −/− |
| Dusp2 | +/+ | + | +/+ |
| Nlk | +/+ | + | −/− |
| Timp3 | +/+ | + | +/+ |
| Dbccr1 | +/+ | − | −/− |
| Klf2 | +/+ | − | −/− |
| Dock4 | +/+ | − | −/− |
| Cacna1g | +/+ | + | −/− |
| Atp1b2 | +/+ | + | +/+ |
| Egr1 | +/+ | − | −/− |
| Smpd3 | +/+ | + | +/+ |
| Akap12 | +/+ | − | −/− |
| Rprm | +/+ | + | +/+ |
| Zfp185 | +/+ | − | −/− |
| Cry2 | +/+ | − | −/− |
| Scrib | +/+ | − | −/− |
| Btg2 | +/+ | − | −/− |
| Foxj1 | +/+ | + | +/+ |
| Sesn3 | +/+ | − | −/− |
| Kremen1 | +/+ | + | −/− |
| Bach2 | +/+ | + | −/− |
| Rb1 | +/+ | − | −/− |
| Ddit3 | +/+ | − | −/− |
| Irf6 | +/+ | − | −/− |
| Bcl2l11 | +/+ | − | −/− |
| Tusc2 | +/+ | ND | ND |
| Tfdp2 | +/+ | ND | ND |
| Ptpn13 | +/+ | ND | ND |
| Ephb3 | +/+ | ND | ND |
| Pcdh10 | +/+ | ND | ND |
| Eaf2 | +/+ | ND | ND |
| Sema3b | +/+ | ND | ND |
| Lrp1b | +/+ | ND | ND |
| Rad9b | +/+ | ND | ND |
| Cdh15 | +/+ | ND | ND |
| Dapk2 | +/+ | ND | ND |
M: mouse, H: human
ND: not done
*
For RT-PCR assays, + = increased gene expression in 5-azadC/TSA treated EMT6 cells
**
For MSP assays, + = gene methylation in any mouse mammary or human breast tumor line studied