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Change in cantilever deflection on exposure to neutravidin. In one case, the silicon nitride surface of the cantilever was coated with biotin and polyethylene glycol, whereas in the other case, the same surface cantilever was coated with a silane layer. Specific binding of neutravidin to biotin generated a negative deflection, indicating generation of compressive stress on the top silicon nitride surface, whereas exposure to silane produced no appreciable deflection signal.
