Table 1.
Oligonucleotide primers (16S rDNA) used to detect fecal bacteria for each PCR assay.
| Primer name and sequence (5′–3′) | Annealing temp (°C) | Product size (bp) | Method |
|---|---|---|---|
| lm26 (GATTCTGGCTCAGGATGAACG) | 55 | 1350 | Bonjoch et al. [12] |
| lm3 (CGGGTGCTICCCCACTTTCATG) | |||
|
| |||
| lm 26 (GATTCTGGCTCAGGATGAACG) | 48 | 777 | King et al. [10] |
| 785R (CTACCAGGGTATCTAATCC) | |||
|
| |||
| BiADO1 (CTCCAGTTGGATGCATGTC) | 55 | 279 | Matsuki [16] |
| BiADO2 (CGAAGGCTTGCTCCCAGT) | Bonjoch et al. [12] | ||
| King et al. [10] | |||
|
| |||
| HuBac566f (GGGTTTAAAGGGAGCGTAGG) | 60 | 116 | Layton et al. [7] |
| HuBac692r (CTACACCACGAATCCGCCT) | |||
*Primer pairs lm26-lm3 and lm26-785R are genus-specific primers for Bifidobacteria.
Primer pair BiADO1 and BiADO2 is species-specific for B. adolescentis
HuBAC566f and HUBAC692r are specific for human associated Bacteroides