Table 3.
Detection of human fecal bacteria (B. adolescentis and HuBAC) PCR Products in environmental samples by multiplex- [10, 12] and single-PCR assays.
| Sample (n) | Matsuki et al. [16] | Bonjoch et al. [12] | King et al. [10] | Layton et al. [7] |
|---|---|---|---|---|
| Raw sewage (3) | 2 | 2 | 3 | 3 |
| Sewage dilution 10−2 on .22 μm filter (3) |
0 | 2 | 3 | 2* |
| Sewage dilution 10−3 on .22 μm filter (3) |
0 | 1 | 3 | 0 |
| Sewage dilution 10−4 on .22 μm filter (3) |
0 | 0 | 3 | 0 |
| Sewage dilution 10−1 on .45 μm filter (3) |
0 | 3 | 3 | 0 |
| Sewage dilution 10−2 on .45 μm filter (3) |
0 | 3 | 3 | 0 |
| Sewage dilution 10−3 on .45 μm filter (3) |
0 | 1 | 3 | 0 |
| Sewage dilution 10−4 on .45 μm filter (3) |
0 | 1 | 3 | 0 |
| Pig (8) | 0 | 3 | 0 | 6 |
| Chicken (4) | 0 | 0 | 0 | 0 |
| Cow (2) | 0 | 0 | 0 | 2* |
| Rabbit (4) | 0 | 0 | 0 | 0 |
| Horse (4) | 0 | 0 | 0 | 1* |
Detection limit was reached in 1/10,000 diluents of sewage.
*Very faint bands