Figure 1.
Amyloid pathology increases the migration of infused GFP monocytes into brain. In A, 5 × 106 GFP bone marrow monocytes were injected into the left ventricle of the heart of nontransgenic mice. At the indicated time points, mice blood was collected and the number of GFP monocytes in the blood was measured by flow cytometry (n = 4 at each time point). In B and C, mice were infused with 5 × 106 GFP+ bone marrow monocytes into the left ventricle of the heart, and organs were collected 1, 3, or 7 d following the injection. The organs were bisected and the number of monocytes present in each organ was quantified using flow cytometry (B) or stereology (C). n = 4 for each organ. D–H are representative micrographs of GFP staining in the tissues indicated above each panel. *p < 0.05 by t test. Scale bar, 25 μm.