Figure 3.

Double labeling of GFP-positive cells migrating into the CNS with markers of the microglial phenotype using fluorescence microscopy. Sections were double immunolabeled for GFP and CD45 (A–C), Iba-1 (D–F), or F4/80 (G–I). The left panel in each series used filters specific for the microglial phenotype marker, the middle panel used filters specific to the GFP cells, and the right panel is a superimposition of the left and middle panels. Yellow appears where there is double labeling of the cells. Magnification, 400×.