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. Author manuscript; available in PMC: 2010 Aug 30.
Published in final edited form as: Biomacromolecules. 2010 Jul 12;11(7):1863–1872. doi: 10.1021/bm100384z

Figure 3. Effect of ZnSO4 on VEGF incorporation in CS-DS PECs.

Figure 3

VEGF-DS-CS PECs were prepared in the absence (A) and presence (B) of 25 μM ZnSO4. VEGF content in the resulting PECs were analyzed by SDS PAGE followed by Coomassie blue staining. Lane 1 in both gels were loaded with MW standards indicating protein sizes of 250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa (from top to bottom). Lane 2, 3, 4, 5, and 6 in both gels were loaded with PECs prepared with 0, 10, 20, 30, and 40 μg VEGF, respectively. The amounts of DS and CS in these preparations were 1 mg and 0.25 mg, respectively.