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. 2010 Aug 2;120(9):3234–3241. doi: 10.1172/JCI42672

Figure 4. Serum cell binding and ADCC assay.

Figure 4

(A) Sera from high-dose cohort patient AVX701-0121 were incubated with CEA-expressing colon tumor cell line SW1463 (CEA+) and non-CEA–expressing colon tumor line COLO320 (CEA–). Results are represented as histograms of MFI for 1:50, 1:100, and 1:200 dilutions. Binding of prevaccination serum antibodies is represented by white peaks, and the postvaccination serum is represented by the gray peaks. The top row of histograms binds against SW1463 (CEA+) cell line and the lower row COLO320 cell line. The ELISA titer for patient AVX701-0121 was 1:200. (B) Serial dilutions of pre- and postimmunization sera from high-dose cohort patient AVX701-0109 were incubated with CEA-expressing colon tumor cell line SW1463 (CEA+) in an ADCC assay. The percentage of specific lysis is presented as mean ± SD. *P < 0.01 for prevaccination versus week 9 serum at 1:20 ratio. The ELISA titer for patient AVX701-0109 was 1:1,600.