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. 2010 Aug 25;120(9):3127–3136. doi: 10.1172/JCI43122

Figure 1. Generation of hepatocytes from disease-specific human iPS cells.

Figure 1

(A) Protocol used to differentiate the disease-specific human iPS cell library into hepatocytes. (B) Immunostaining analyses for expression of the indicated proteins marking key stages of hepatocyte development (day 4, endoderm; day 20, hepatic progenitor; day 25, fetal hepatocyte). (C) Real-time PCR analysis for expression of genes marking key stages of disease-specific human iPS cell (hIPSC) differentiation to hepatocytes. Error bars denote SEM. (D) Fraction of cells expressing albumin after 25 days of hepatic differentiation, as shown by FACS analyses. (E) Morphologic analysis of disease-specific human iPS cell–derived hepatocytes (day 25) by transmission EM, showing the presence of apical microvilli and glycogen rosettes (numerals 1 and 2, respectively). Original magnification, ×20 (A); ×40 (B); ×3,000 (E). The data shown were taken from 1 line (patient 1; line 1), but are representative of all lines similarly characterized (Table 1).