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. Author manuscript; available in PMC: 2010 Aug 30.
Published in final edited form as: Epigenetics. 2009 Feb 23;4(2):100–106. doi: 10.4161/epi.4.2.7953

Figure 3.

Figure 3

H3K4me3 demethylase activity of the ZNF217 complex correlating with Jarid1b/Plu-1. The affinity-purified ZNF217 protein complex was fractionated on a MonoQ column. Individual fractions were tested for demethylase activity by incubation with H3K4me3 substrate. Western blotting with an H3K4 trimethyl-specific antibody demonstrates loss of methylation, i.e., presence of demethylase activity in fractions 6–10. This correlates with the presence of Jarid1/Plu-1 indicated in the corresponding silver stained SDS gel.