TABLE 1.
COMPARISON OF IMMOBILISATION EFFICIENCY AND WORKING EFFICIENCY (%) OF BEADS
| Gel concentration (%) | Immobilization effi ciency (%) on the basis of amylase activity in the supernatant |
Working effi ciency (%) of beads on the basis of enzyme activity |
||
|---|---|---|---|---|
| Agarose gel | Calcium alginate | Agarose gel | Calcium alginate | |
| 0.25 | 45-50 | 93-95 | 33 | 85 |
| 0.50 | 65-70 | 98-99 | 59 | 90 |
| 1.0 | 88-92 | 98-99 | 78 | 93 |
| 1.5 | 88-93 | 98-99 | 88 | 86 |
| 2.0 | 93-95 | 98-99 | 78 | 81 |
Enzyme activity was taken after washing with 0.1 M phosphate buffer (pH 7.0) thrice to remove loosely bound enzyme. These values are mean of six observations