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. 2010 Mar-Apr;72(2):223–228. doi: 10.4103/0250-474X.65010

TABLE 2.

COMPARISON OF IMMOBILISATION EFFICIENCY AND WORKING EFFICIENCY (%) OF BEADS

Gel concentration (%) Immobilization effi ciency (%) on the basis of amylase activity in the supernatant Working effi ciency (%) of beads on the basis of enzyme activity
Polyacrylamide gel Polyacrylamide gel
7.5 78-82 59
10.0 88-92 67
12.5 92-95 79
15.0 93-95 89
20.0 97-99 75

Enzyme activity was taken after washing with 0.1 M phosphate buffer (pH 7.0) thrice to remove loosely bound enzyme. These values are mean of six observations