TABLE 2.
Gel concentration (%) | Immobilization effi ciency (%) on the basis of amylase activity in the supernatant | Working effi ciency (%) of beads on the basis of enzyme activity |
---|---|---|
Polyacrylamide gel | Polyacrylamide gel | |
7.5 | 78-82 | 59 |
10.0 | 88-92 | 67 |
12.5 | 92-95 | 79 |
15.0 | 93-95 | 89 |
20.0 | 97-99 | 75 |
Enzyme activity was taken after washing with 0.1 M phosphate buffer (pH 7.0) thrice to remove loosely bound enzyme. These values are mean of six observations