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. Author manuscript; available in PMC: 2011 Jun 30.
Published in final edited form as: J Am Chem Soc. 2010 Jun 30;132(25):8676–8681. doi: 10.1021/ja101384k

Table 1.

Thermodynamic data for binding of PNA to RNA hairpin HRP1.a

Entry Sequence Ka

× 107 M−1 		−ΔH

kcal/mol		 −ΔS

eu		 −ΔG

kcal/mol		 Binding
order
1 RNA 5’-CCUCUCCUCCCU 1.3 56.1 156 9.7 0.8
2 PNA1 NH2-CCTCTCCTCCCT 35 ± 30 55.4 ± 5.3 147 ± 17 11.5 ± 0.6 1.2 ± 0.1
3 PNA2 NH2-TCCCTCCTCTCC 4.5 42.5 108 10.4 1.3
4 PNA3 NH2-CTCCTC 8.4 ± 8 29.4 ± 5.3 63 ± 17 10.6 ± 0.5 1.1 ± 0.2
5 PNA1 b NH2-CCTCTCCTCCCT 8.7 ± 4 86.6 ± 3.9 254 ± 13 10.8 ± 0.1 0.74 ± 0.01
6 PNA1 c NH2-CCTCTCCTCCCT 0.05 36.3 96 7.7 1.0
a

Association constants Ka (average ± standard deviation) in 100 mM sodium acetate, 1.0 mM EDTA, pH 5.5.

b

Reverse order of titration – RNA is added via syringe to PNA in calorimeter cell.

c

Same buffer at pH 7.