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. Author manuscript; available in PMC: 2011 Jul 20.
Published in final edited form as: Langmuir. 2010 Jul 20;26(14):12140–12146. doi: 10.1021/la101674b

Figure 4.

Figure 4

The adsorption of IgG onto the gradient surface.

(A): Alexa 488-IgG adsorption-desorption kinetics measured in a TIRF experiment and calibrated using autoradiography data from Fig. 4B.

(B): Adsorption of 125I-IgG along the sulfhydryl-to-sulfonate gradient surface recorded by autoradiography at 1/100 (top), 1/500 (middle), and 1/1000 (bottom) plasma protein concentration. Top – autoradiography images of 125I-IgG adsorbed onto the whole length of a MTS modified FS slides with the gradient region in the middle of the slide as indicated. Bottom – calibrated 125I-IgG adsorption profiles along the gradient region.