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. Author manuscript; available in PMC: 2010 Aug 30.
Published in final edited form as: Mol Cancer Res. 2008 Nov;6(11):1755–1765. doi: 10.1158/1541-7786.MCR-08-0095

Figure 2.

Figure 2

Figure 2

Figure 2

S-phase cyclin E and its binding partner CDK2 are up-regulated in MIA-MSLN cells. A. Sub-confluent cells were used to prepare lysates, and 60 µg of protein were subjected to SDS-PAGE and Western blot. Various cell-cycle-related proteins were detected with antibodies mentioned in methods.B. Control cells and MIA-MSLN cells were serum-starved (0% FBS) for 24 h, changed to 2% FBS medium, and collected at indicated times, and whole cell proteins were subjected to SDS PAGE, Western blotting, and probing for cyclin E, CDK2, and β actin. C. 400 µg of MIA-V and MIA-MSLN proteins was used to immunoprecipitate CDK2 by using immobilized protein G-conjugated-anti-CDK2 monoclonal antibody, and the immune-complex precipitate was washed and subjected to SDS-PAGE under denaturing conditions, gel-transferred to nitrocellulose membrane, and probed for cyclin E and CDK2.