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. Author manuscript; available in PMC: 2010 Aug 30.

Published in final edited form as: Mol Cancer Ther. 2008 Feb;7(2):286–296. doi: 10.1158/1535-7163.MCT-07-0483

Fig. 6 — Reduced numbers of Tregs in VLP-hMSLN-vaccinated mice. About 5 × 10⁵ Panc02 cells were implanted into the body of pancreases of C57BL/6J wild-type mice. On day 3, 50 μg VLP-hMSLN, VLPGag, or PBS was injected intraperitoneally (10 mice in each group); this was followed on day 7 and day 14 with two 100-μg VLP-hMSLN, VLP-Gag, or PBS boosts. Two weeks after the final VLP boost, mouse splenocytes were collected and stained for CD4, CD25, and Foxp3 expression by flow cytometry (A.); or mouse Panc02 tumor sections were stained with Foxp3 Ab to visualize number of Treg cells (red cells) in tumors with PBS, VLP-Gag, or VLP-hMSLN immunization (B.). The number of Tregs was lower in VLP-hMSLN vaccinated mice. All data shown are representatives of three separate experiments.

Fig. 6 — Reduced numbers of Tregs in VLP-hMSLN-vaccinated mice. About 5 × 10⁵ Panc02 cells were implanted into the body of pancreases of C57BL/6J wild-type mice. On day 3, 50 μg VLP-hMSLN, VLPGag, or PBS was injected intraperitoneally (10 mice in each group); this was followed on day 7 and day 14 with two 100-μg VLP-hMSLN, VLP-Gag, or PBS boosts. Two weeks after the final VLP boost, mouse splenocytes were collected and stained for CD4, CD25, and Foxp3 expression by flow cytometry (A.); or mouse Panc02 tumor sections were stained with Foxp3 Ab to visualize number of Treg cells (red cells) in tumors with PBS, VLP-Gag, or VLP-hMSLN immunization (B.). The number of Tregs was lower in VLP-hMSLN vaccinated mice. All data shown are representatives of three separate experiments.