Figure 2.

Coherent anti-Stokes Raman scattering microscopy of D. discoideum. (a) CARS image of a single dictyostelium cell in aqueous medium. Pump laser was tuned to 636 nm, and Stokes beam was set to 800 nm to address the O—H-stretch vibration of water. The image is recorded in 5 s and consists of 16 averages per pixel. (Bar = 2 μm.) (b) Autofluorescence image measured simultaneously with a.