Figure 1.
Hepatic MMP-9 mRNA and protein expressions in septic rats: Liver tissues from sham rats, and rats subjected to sepsis by CLP for 10h and 20 h were examined for MMP-9. (A) MMP-9 mRNA expression was assessed by quantitative real time PCR using primers specific for rat MMP-9. Results are shown as fold change over GAPDH levels. (B) MMP-9 protein levels were measured by Western blotting using anti-MMP-9 antibodies. Antibody to β-actin is used as the internal control. Results are shown as MMP-9/β-actin ratio in arbitrary densitometric units. Data are presented as mean ± SE (n=4) and compared by oneway analysis of variance and Student-Newman-Keuls method: *P < 0.05 versus Sham group.