Figure 3.

Hepatic TIMP-1 mRNA and protein expressions in septic rats: Liver tissues from sham and septic rats were examined for TIMP-1. (A) TIMP-1 mRNA expressions were determined by quantitative real time PCR using primers specific for TIMP-1 and results are expressed as fold change over GAPDH. (B) TIMP-1 protein levels were assessed by Western blotting using anti-TIMP-1 antibodies. Results are shown as TIMP-1/β-actin ratio. Data are presented as mean ± SE (n=4) and compared by one-way analysis of variance (ANOVA) and Student-Newman-Keuls method. *P < 0.05 versus Sham group.