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Assessments of nicotine and cotinine levels in CCS-exposed PBS. Two milliliters of PBS in each 35-mm dish were exposed to air (0 EU), or 1-4 EU (4-16 cigarettes) of CCS as described in the Materials and Methods. The CCS-exposed PBS was collected and subjected to LC-MS/MS analysis. The levels of nicotine (Fig 2A) and cotinine (Fig 2B) were determined. Data represent the means ± SD of triplicates. *:P<0.05vs. 1EU, n=3.
