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The correlation of OD290nm and nicotine levels. PBS (2 mL) in dishes (35-mm) was exposed to air (0 EU), or 1-4 EU (4-16 cigarettes) of CCS as described in the Materials and Methods. The OD290nm values of the exposed PBS was assessed (Fig 4A). The correlation of OD290nm values and nicotine levels in CCS-exposed PBS is shown (Fig 4B). Data represent the means ± SD of triplicates. *: P<0.05 vs. 1 EU, n=3.
