Figure 2. SNS-032 inhibited transcription in the MCL cell lines.
A. SNS-032 inhibited phosphorylation of CTD of RNA pol II in MCL cells. MCL cells were incubated with indicated concentrations of SNS-032 for 6 and 24 hr. The phosphorylation of RNA pol II at Ser2 and Ser5 sites as well as total pol II protein were studied by immunoblotting. B. SNS-032 inhibited RNA synthesis in the MCL cells, measured by [3H]uridine incorporation at 6 (○) and 24 h (■) after incubation with a series of concentrations of SNS-032, and presented as percentage of controls from three to six triplicate experiments.