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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Cancer Res. 2010 Jul 27;70(16):6587–6597. doi: 10.1158/0008-5472.CAN-09-3578

Figure 5. Effect of siRNA of Mcl-1 and cyclin D1 in JeKo-1 cells.

Figure 5

JeKo-1 cells were transfected with siRNAs of control siRNA (ctrl), Mcl-1, cyclin D1 (CycD1) or the combination of both siRNAs (Mcl-1+CycD1). A. Quantitation of Mcl-1 and cyclin D1 protein levels at 24 (white bars) and 48 h (grey bars) after transfection, expressed as the mean +/− SD of two independent experiments. B. A representative immunoblot of protein expression at 48 h after transfection. C. Transfection of Mcl-1 siRNA significantly reduced JeKo-1 cell survival. Cell viability was measured at 48 h after transfection by Annexin V/PI staining. Data represent mean percentage +/− SD of live cells after transfection from three independent experiments. * indicates significantly different from control siRNA transfected group. p = 0.005 for Mcl-1; p= 0.03 for Mcl-1+CycD1.