Skip to main content
. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Cancer Res. 2010 Jul 27;70(16):6587–6597. doi: 10.1158/0008-5472.CAN-09-3578

Figure 6. Effect of siRNA of Mcl-1 and cyclin D1 in Granta 519 cells.

Figure 6

Granta 519 cells were transfected with siRNAs of control siRNA (ctrl), Mcl-1, cyclin D1 (CycD1) or the combination of both siRNAs (Mcl-1+CycD1). A. Quantitation of Mcl-1 and cyclin D1 protein levels at 24 (white bars) and 48 h (grey bars) after transfection, expressed as the mean +/− SD of three independent experiments. B. A representative immunoblot of protein expression at 48 h after transfection. C. Neither siRNA of Mcl-1 nor cyclin D1 affected short-term survival of Granta 519 cell. Cell viability was measured at 24 (white bars) and 48 h (grey bars) after transfection by Annexin V/PI staining. Data represent mean percentage +/− SD of live cells after transfection from three independent experiments. D. Cyclin D1 siRNA significantly reduced clonogenic survival of Granta 519 cells. 6 hr after transfection, the cells were seeded onto methylcellulose media for cloning. Data represent percentage of colonies of control siRNA-transfected cells of three independent experiments performed in triplicates. * Significantly different from control siRNA-transfected group. p = 0.006 for CycD1; p= 0.009 for Mcl-1+CycD1.