Table 1.
Determination of expression levels of genes involved in lipid metabolism in tunicamycin- or vehicle-injected ATF6α +/+ and −/− mice by microarray analysisa
| Gene name | Basal (%) KO vehicle WT vehicle | Change in WT (%) Tm Vehicle | Change in KO (%) Tm Vehicle | Ratio (%) Change in KO Change in WT | GenBank |
|---|---|---|---|---|---|
| FA uptake | |||||
| FABPm/AST | 92 | 88 ± 7.1 | 90 ± 6.1 | 100 | NM_010325 |
| Fatty acid translocase/CD36 | 90 | 93 ± 31 | 30 ± 3.7 | 32 | L23108 |
| FATP/Slc27al | 75 | 52 ± 17 | 32 ± 7.0 | 62 | NM_011977 |
| FA activation | |||||
| ACSL1 | 110 | 61 ± 2.6 | 7.6 ± 1.6 | 12 | NM_007981 |
| FABP1 | 170 | 123 ± 57 | 0.35 ± 0.17 | 0.28 | NM_017399 |
| FATP2/Slc27a2 | 120 | 101 ± 5.2 | 5.7 ± 2.6 | 5.6 | NM_011978 |
| FATP5/Slc27a5 | 110 | 82 ± 32 | 6.3 ± 1.0 | 7.7 | NM_009512 |
| FA β-oxidation | |||||
| PPARα | 50 | 56 ± 12 | 14 ± 3.8 | 25 | NM_011144 |
| CPT-Ia | 83 | 101 ± 11 | 42 ± 4.2 | 42 | NM_013495 |
| CPT-II | 110 | 60 ± 9.3 | 16 ± 5.6 | 27 | NM_009949 |
| ACOX1 | 130 | 215 ± 29 | 32 ± 10 | 15 | NM_015729 |
| FA synthesis | |||||
| SREBP-1 | 64 | 30 ± 14 | 6.8 ± 2.9 | 23 | NM_011480 |
| Fatty acid synthase | 97 | 12 ± 2.2 | 4.4 ± 1.9 | 37 | NM_007988 |
| Stearoyl-CoA desaturase1 | 70 | 9.6 ± 2.9 | 7.8 ± 1.6 | 81 | NM_009127 |
| ChREBP | 83 | 72 ± 23 | 8.9 ± 3.3 | 12 | NM_021455 |
| PPARγ | 70 | 93 ± 11 | 112 ± 15 | 120 | NM_011146 |
| PPRC1 | 61 | 107 ± 24 | 454 ± 61 | 420 | BC066048 |
| LD formation | |||||
| ADRP | 45 | 132 ± 44 | 966 ± 442 | 730 | NM_007408 |
| TIP47 | 81 | 89 ± 7.1 | 450 ± 280 | 510 | NM_025836 |
| FA export | |||||
| MTP | 95 | 82 ± 2.6 | 30 ± 9.0 | 37 | NM_008642 |
| Apolipoprotein | |||||
| Apolipoprotein A-I | 73 | 73 ± 33 | 3.0 ± 5.9 | 4.1 | NM_009692 |
| Apolipoprotein A-II | 193 | 125 ± 46 | 6.9 ± 1.9 | 5.5 | NM_013474 |
| Apolipoprotein A-V | 300 | 98 ± 21 | 3.6 ± 0.40 | 3.7 | NM_080434 |
| Apolipoprotein B | 120 | 122 ± 30 | 15 ± 8.0 | 12 | AK147540 |
| Apolipoprotein C-I | 120 | 93 ± 18 | 19 ± 10 | 20 | NM_007469 |
| Apolipoprotein C-II | 110 | 120 ± 29 | 17 ± 2.2 | 14 | NM_009695 |
| Apolipoprotein C-III | 73 | 38 ± 4.2 | 6.8 ± 1.1 | 18 | NM_023114 |
| Apolipoprotein E | 110 | 104 ± 15 | 22 ± 9.4 | 21 | NM_009696 |
| Cholesterol metabolism | |||||
| SREBP-2 | 91 | 41 ± 8.9 | 11 ± 2.5 | 27 | AK172219 |
| HMG-CoA synthase1 | 100 | 22 ± 7.9 | 13 ± 7.7 | 59 | NM_145942 |
| HMG-CoA synthase2 | 72 | 77 ± 16 | 11 ± 3.7 | 14 | NM_008256 |
| HMG-CoA reductase | 86 | 14 ± 7.3 | 6.1 ± 1.3 | 44 | AK159899 |
| LDL receptor | 75 | 26 ± 8.1 | 3.8 ± 6.9 | 15 | BC053041 |
| LRP1 | 96 | 72 ± 11 | 8.8 ± 2.3 | 12 | NM_008512 |
a Aray analysis was conducted using total RNA independently prepared three times from livers 48 h after intraperitoneal injection of tunicamycin or vehicle (150 mM sucrose) alone into ATF6α +/+ (WT) and −/− (KO) mice. Basal (%), change in WT (%), change in KO (%), and ratio (%) were determined as indicated at the top. Gene accession numbers also are shown.