Figure 5.

The 3′-UTR of AIP-1 confers QKI-6 sensitivity to a heterologous RNA. (A) Schematic illustration of pMIR-reporter vectors with or without the 3′-UTR of AIP-1, as well as with a mutated QRE version of the AIP-1 3′-UTR. (B) HEK293 cells were cotransfected with pcDNA, pcDNA-QKI-6, or pcDNA-QKI-6:V157E, along with the indicated pMIR-reporter vectors and pTKrenilla to control for transfection efficiency. The luciferase activity was expressed as a normalized ratio of the activity of luciferase/Renilla (± represents the SE of the mean). Each bar represents three separate experiments. One-way analysis of variance was performed in comparison with absence of QKI-6 transfection. Single asterisk denotes a p < 0.005.