Figure 7.

AIP-1 expression decreases during primary rat OL differentiation. (A) Primary rat OPCs were induced to differentiate for the indicated days, and the cells were lysed and protein extracts were separated by SDS-polyacrylamide gel electrophoresis. The presence of α-tubulin, AIP-1, and MBP isoforms were analyzed by immunoblotting. α-Tubulin levels were used to confirm equivalent loading, and MBP expression was used as a marker for OL differentiation. Total RNA levels were isolated from OLs differentiated for different time points (day 0–4) and analyzed by real-time PCR for changes in mRNA levels of AIP-1 (B), MBP (C), and Id2 (D). Real-time data represent three separate experiments. One-way analysis of variance was performed in comparison with day 0 samples; single asterisks denote a p < 0.05 and double asterisks denote a p < 0.005. RNA half-life for Actin (E) and AIP-1(F) was determined by actinomycin D treatment for 0–24 h and compared between different time points of OL differentiation (day 0 and day 4). Real-time data represent three separate experiments. Two-way analysis of variance was performed in comparison with day 0 OPCs; double asterisks denote a p < 0.005.