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. 2010 Sep 1;21(17):3054–3069. doi: 10.1091/mbc.E10-03-0181

Figure 1.

Figure 1.

Rvs161-Rvs167 binds and tubulates liposomes in vitro. (A) Schematic diagram showing domain structures of Rvs161 and Rvs167. Rvs161 and Rvs167 form a heterodimer via their BAR domains. (B) Coomassie-stained SDS-polyacrylamide gel of lipid cosedimentation assays. Purified Rvs161-Rvs167 at 2 μM was incubated with Folch (brain) liposomes with two different ranges of curvature, and the mixture was subjected to ultracentrifugation to separate the liposome-bound fraction (pellet, P) and the unbound fraction (supernatant, S). (C) Electron micrographs of synthetic liposomes (containing yeast-membrane composition; see Materials and Methods) incubated with 3 μM Rvs161-Rvs167. Liposomes form tubules ∼18–20 nm in diameter (arrow). Scale bar, 200 nm.