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. 2010 Sep 1;21(17):3054–3069. doi: 10.1091/mbc.E10-03-0181

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 5. — Localization of Rvs167-GFP in rvs161 rvs167 mutants. (A) Maximum intensity projections of Z-stacks of C-terminally GFP-tagged Rvs167 strains. The signal for Rvs167-AP-GFP is two-fold enhanced to show its cortical localization. Scale bar, 4 μm. (B) Single images of Rvs167-GFP, Rvs167-RC-GFP, and Rvs167-AP-GFP with actin patch marker, Abp1-mCherry. Scale bar, 4 μm. (C) Rvs167-GFP patch density [patch number/cell surface area (μm²) ± SD] in wild-type and rvs161-RC rvs167-RC cells (n ≥ 100, three independent repeats). (D) Rvs167-RC-GFP exhibits a specific defect in its internalization step. On the left, single frames from live-cell movies show localization of Rvs167-GFP and Rvs167-RC-GFP. The corresponding kymographs are shown on the right. Movies corresponding to these kymographs are in Supplementary Movie 1 and 2. Each kymograph shows the dynamics of two or four patches on opposite sides of a cell over 120 s. Scale bar, 2 μm.