Table 1.
Summary of rvs BAR domain mutant phenotypes
| rvs161 rvs167genotype | Cell fusion (mating) | Growth on salta | LY uptakeb | Sla1-GFP internalizationc | Localization to coritcal actin patches (Rvs167-GFP)d | Synthetic growth defect in inp52Δ background | AbA sensitivitye |
|---|---|---|---|---|---|---|---|
| WT WT | + | + | + | + | + | + | + |
| Δ Δ | − | − | − | − | − | − | − |
| ΔNΔN | − | −f | − | ± | − | − | −f |
| RC RC | + | − | ± | − | + | − | − |
| AP AP | − | + | + | ± | + | + | − |
| KE(con) KE(con) | ± | − | − | − | − | − | − |
| KE(loop) KE(loop) | ± | − | ± | − | ± | + | − |
| KE(con+loop) KE(con+loop) | − | − | − | − | − | − | − |
a Growth sensitivity on 0.7 M NaCl-containing medium was used to assess growth. +, colonies form in three or more serially diluted spots; −, colonies form in ≤2 spots.
b +, more than 60% of cells show LY-stained vacuole; ±, between 30 and 60% of cells show LY-stained vacuole; −, <30% of cells show LY-stained vacuole.
c +, <5% of Sla1-GFP patches fail to internalize and show retraction; ±, between 5 and 13% of the Sla1-GFP patches fail to internalize and show retraction; −, between 13 and 22% of the Sla1-GFP patches fail to internalize and show retraction.
d ±, Rvs167-GFP patch density is reduced; −, no punctate RVS167-GFP patches detected.
e Growth in 0.1 μg/ml AureobasidinA (AbA)-containing media. +, colonies form in three or more serially diluted spots; −, colonies form in ≤2 spots.
f ΔN ΔN phenotype is more severe than Δ Δ.